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AMPK: Methods and Protocols: 1732 (Methods in Molecular Biology, 1732)
Item #: 274290458

AMPK: Methods and Protocols: 1732 (Methods in Molecular Biology, 1732)

Item #: 274290458
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Product Details

Shop AMPK: Methods and Protocols: 1732 (Methods in Molecular Biology, 1732) online at a best price in Turkey. 1493975978
  • This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.
Publisher Humana
Publication date 26 Feb. 2018
Edition 2018th
Language English
Print length 643 pages
ISBN-10 1493975978
ISBN-13 978-1493975976
Item weight 1.32 kg
Dimensions 17.78 x 3.51 x 25.4 cm

Product Description

AMPK: Methods and Protocols: 1732 (Methods in Molecular Biology, 1732)

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